RESEARCH LIBRARY • NASAL PREPARATION

Nasal Format Preparation Reference

A step-by-step reference for preparing nasal-format research peptides.

Nasal-format research peptides are reconstituted in the vial, then transferred to a nasal spray bottle and diluted to the final volume. This reference covers the materials required, the full preparation procedure, and storage conditions.

What you will need

Your peptide vial (lyophilized powder)
Bacteriostatic water (BAC) — sterile water with 0.9% benzyl alcohol
Sterile syringe
Alcohol wipes for sterilization
Glass 1 oz nasal sprayer (0.1 mL per spray)

Before you begin

Use a new syringe and needle for each peptide preparation.
Work in a clean area and let wiped surfaces air-dry.

Step-by-step preparation

Clean surfaces
- Wipe the rubber tops of the peptide vial and BAC vial with alcohol swabs.
- Wipe the nasal bottle opening too.
Reconstitute in the vial
- Draw 2 mL of BAC water into the syringe.
- Slowly inject it into the peptide vial, aiming along the side wall to reduce foaming.
- Gently swirl or roll the vial until the powder fully dissolves — it should go clear quickly. Avoid vigorous shaking.
Transfer to the nasal bottle
- Draw the full 2 mL solution back into the syringe.
- Inject it into your empty nasal spray bottle.
Add the remaining BAC
- Draw another 3 mL of BAC water.
- Add it to the nasal spray bottle. Total volume is now ~5 mL.
Mix and prime
- Gently swirl or shake the bottle to mix evenly.
- Prime the pump: spray 2–3 times into the air (or a tissue) until you get a consistent fine mist.

Bacteriostatic water and a Semax research vial being reconstituted with a sterile syringe — Injecting BAC water slowly down the side of the vial wall to reduce foaming.

Transferring reconstituted peptide solution into an amber nasal spray bottle — Transferring the reconstituted solution into the nasal spray bottle. An amber bottle helps protect from light.

Storage and usage notes

Refrigerate immediately (2–8°C / 36–46°F). Do not freeze.
With BAC, it should stay stable for up to 4 weeks. Some go 30+ days if no cloudiness or odd smell appears — check visually before each use.
Keep away from light (an amber bottle helps) and avoid frequent temperature swings.

Detail	Reference
Final volume	~5 mL (2 mL reconstitution + 3 mL dilution)
Spray size	0.1 mL per spray
Storage	Refrigerated, 2–8°C (do not freeze)
Stability	Up to ~4 weeks with BAC

Related research

Nasal format is commonly used with intranasal research peptides. See the compound pages for research details and Evidence Snapshots.

Semax → Selank → Oxytocin → General reconstitution reference →

Need supplies?

Research peptides, bacteriostatic water, and nasal sprayers are available at BLP Research.

Visit BLP Research →

Research use only

This reference is provided for laboratory and research purposes only. All materials are sold and offered strictly for research use and are not intended to diagnose, treat, cure, or prevent any disease. Statements have not been evaluated by the Food and Drug Administration.